FastQCFastQC Report
Thu 25 Aug 2022
EGAF00005312291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35937910
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3077580.8563603170022964Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2689550.7483879836083956Illumina Single End PCR Primer 1 (100% over 50bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA1079370.3003430082606362No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)