FastQCFastQC Report
Sat 27 Aug 2022
EGAF00005312296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41071353
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTATGCAATCTCGTAT5331901.29820412782603TruSeq Adapter, Index 8 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTATGCAATCTCGTA5181251.2615240603347058TruSeq Adapter, Index 8 (97% over 36bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG3688740.8981296525585608No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT2054600.5002513552451023No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA1707880.41583241730556086No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)