FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40625213
Sequences flagged as poor quality0
Sequence length100
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA6514671.6036026691109286No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3868350.9522042382891629Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3480000.8566108933385778Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG1327490.32676505597644495No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT643200.15832532373430264No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)