FastQCFastQC Report
Thu 25 Aug 2022
EGAF00005312310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43404404
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTTCGAATCTCGTAT1900390.4378334511861976TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTTCGAATCTCGTA1697980.3911999344582637TruSeq Adapter, Index 7 (97% over 35bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG759420.17496381242788175No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA439210.10119019258967361No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)