FastQCFastQC Report
Sat 27 Aug 2022
EGAF00005312314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48130809
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACCAAATCTCGTA3158960.6563280496698071TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACCAAATCTCGTAT2858660.59393558084594TruSeq Adapter, Index 22 (97% over 38bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG1428190.2967309358959664No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA797950.16578778054613627No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT643490.1336960698084256No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)