FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21241902
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCTGAATCTCGTA3175551.4949461681915301TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCTGAATCTCGTAT2856061.3445406159956863TruSeq Adapter, Index 7 (97% over 35bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG1321410.6220770625907228No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT735120.34607070496794495No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA640990.30175734734111853No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACAC238390.11222629687303895Illumina Single End PCR Primer 1 (100% over 33bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229360.10797526511514835No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)