FastQCFastQC Report
Sat 27 Aug 2022
EGAF00005312333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46635114
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2447210.5247569460214035Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2384920.5114000579048654Illumina Single End PCR Primer 1 (100% over 50bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA1857940.3983993691963528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)