FastQCFastQC Report
Sat 27 Aug 2022
EGAF00005312340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44439729
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG4909901.1048447212628143No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTAGAATCTCGTA3635910.8181665554261144TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTAGAATCTCGTAT3551230.7991115337359505TruSeq Adapter, Index 20 (97% over 37bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA2558770.5757843392789367No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT2550360.5738918884946396No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT502290.11302724190779831No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)