FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53901794
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA4869420.9033873714852607No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1876630.3481572431522409Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1504310.2790834754034346Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG776070.14397851025143987No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)