FastQCFastQC Report
Sat 27 Aug 2022
EGAF00005312378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46545064
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGCGACATCTCGTA2867840.616142669822089TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGCGACATCTCGTAT2722640.5849470955717238TruSeq Adapter, Index 3 (97% over 37bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG1657880.35618814489115325No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA757560.16275839689467395No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT724560.1556684936559546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)