FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51954063
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG34414706.6240632614238475Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC25854744.976461609941844Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTAGGGAAAGAGTGTAGATCGGAAGAGCGTCG3603230.6935415234030878No Hit
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCGTC2637410.5076426842689858Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGATCGGAAGAGCACACGTCTAGGGAAAGAGTGTAGATCGGAAGAGCGTC1034250.19907009005243728Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCGTCG944040.18170667422103254Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTGATCGGAAGAGCGTCG766260.1474879837598072Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTGATCGGAAGAGCACAC708710.13641089052072788Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)