FastQCFastQC Report
Sat 27 Aug 2022
EGAF00005312414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38865367
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCCTAAATCTCGTAT17134614.408709172873628TruSeq Adapter, Index 27 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCCTAAATCTCGTA10048672.585507554836675TruSeq Adapter, Index 27 (97% over 39bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCACAC1651410.424905289071373Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACAC618110.15903876579886664Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTACACTCTTTCCCTAGA500240.12871099351769918Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCCTAAATCTCGTAT489710.1260016404836728TruSeq Adapter, Index 15 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)