FastQCFastQC Report
Mon 5 Sep 2022
EGAF00005312418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48021112
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTAT37560307.821622289796204TruSeq Adapter, Index 1 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTA22798564.747611842058134TruSeq Adapter, Index 1 (97% over 38bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCACAC3941060.8206931984415522Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACAC1686720.35124551051629127Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCACA840940.17511881024329465Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTACACTCTTTCCCTAGA749400.15605636121046093Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTACCTCGTAT746870.15552950960402584TruSeq Adapter, Index 1 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTAACTCGTAT703220.14643975757995775TruSeq Adapter, Index 1 (97% over 38bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTACAC655600.13652328584144408Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG630480.13129225329059435Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTAGCTCGTAT492600.10257988194858961TruSeq Adapter, Index 1 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)