FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32468130
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACATTGGCATCTCGTAT18129755.583860234636242TruSeq Adapter, Index 5 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACATTGGCATCTCGTA8683712.674533457886241TruSeq Adapter, Index 5 (97% over 39bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCACAC2282810.7030925402848885Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTACAC1148410.3537037704358089Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTACACTCTTTCCCTAGA930450.28657332590451007Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACACATTGGCATCTCGTAT787140.2424346582325499TruSeq Adapter, Index 5 (97% over 36bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACAC702890.21648613578915693Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTAGAC590630.1819106921156223Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCACA447960.13796914081593242Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
NGATCGGAAGAGCACACGTCTGAACTCCAGTCACACATTGGCATCTCGTA378040.11643417714540381TruSeq Adapter, Index 5 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACATTGGCACCTCGTAT343200.10570365462994019TruSeq Adapter, Index 5 (97% over 39bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)