FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39696545
Sequences flagged as poor quality0
Sequence length100
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG8416802.1202852792352584Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC6846971.724827689664176Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTAGGGAAAGAGTGTAGATCGGAAGAGCGTCG601030.15140612363116235No Hit
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCGTC552270.13912293878472295Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
NATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG484640.12208619163204253Illumina Single End PCR Primer 1 (98% over 50bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA403950.101759485617703No Hit
NGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC397800.1002102324018375Illumina Single End PCR Primer 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)