FastQCFastQC Report
Thu 25 Aug 2022
EGAF00005312436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55171559
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAATCTCGTAT40977197.427230758514545TruSeq Adapter, Index 1 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAATCTCGTA32820705.948844041184335TruSeq Adapter, Index 1 (97% over 37bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCACAC4486950.8132722876292112Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACAC3739200.677740500318289Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTACAC1517040.27496776010987833Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCACA1346650.24408409412538082Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTACACTCTTTCCCTAGA1165740.21129364859890945Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG893310.1619149460684988Illumina Single End PCR Primer 1 (96% over 32bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTGATCGGAAGAGCACACG870270.15773888136820638Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTAGAC769480.13947041083250883Illumina Single End PCR Primer 1 (100% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAAACTCGTAT700830.12702740555147263TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAACCTCGTAT664770.1204914292887754TruSeq Adapter, Index 1 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAAACTCGTA583660.10579001401791092TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTGAACTCCAG568810.10309840981655059Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTGATCGGAAGAGCACAC552560.10015305168374887Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)