FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35290012
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTAT23747156.729141945318692TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTA18905085.3570625025573815TruSeq Adapter, Index 1 (97% over 36bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCACAC2423200.6866532094123402Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACAC1814230.5140916358996988Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTACAC800860.2269367321269259Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCACA745660.21129491256619576Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTACACTCTTTCCCTAGA545110.15446580182517364Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAAACTCGTAT521990.14791437305263597TruSeq Adapter, Index 1 (97% over 36bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG479170.13578062824121453Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAACCTCGTAT449500.1273731502273221TruSeq Adapter, Index 1 (97% over 36bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTGATCGGAAGAGCACACG432170.12246241231088276Illumina Single End PCR Primer 1 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAAACTCGTA432000.12241424004049645TruSeq Adapter, Index 1 (97% over 36bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTAGAC408310.11570129247901643Illumina Single End PCR Primer 1 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAACCTCGTA356850.10111926286678508TruSeq Adapter, Index 1 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)