FastQCFastQC Report
Thu 25 Aug 2022
EGAF00005312446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57825290
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGCTTAATCTCGTAT53136899.189212885918947TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGCTTAATCTCGTA41520087.180263168589383TruSeq Adapter, Index 1 (97% over 36bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCACAC6631391.1467975344351926Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACAC4336380.7499106359864344Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTACAC3266790.5649413950193765Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTACACTCTTTCCCTAGA2173780.3759220230456259Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCACA1788370.3092712548436852Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTAGAC1682500.29096265665074916Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG1209060.20908844555729855Illumina Single End PCR Primer 1 (96% over 32bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTGATCGGAAGAGCACACG1034690.17893381944128597Illumina Single End PCR Primer 1 (100% over 33bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG814930.1409296866474859No Hit
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTGATCGGAAGAGCACAC798730.13812814427735684Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGCTTAAACTCGTAT791700.1369124132364922TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGCTTAACCTCGTAT784550.1356759300299229TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTGAACTCCAG726140.1255748133731798Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGCTTAACCTCGTA660880.11428909392412903TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGCTTAAACTCGTA637510.11024760965314657TruSeq Adapter, Index 1 (97% over 36bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA593400.10261945940954209No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)