FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41789984
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACGGAATCTCGTAT20691914.951404145069785TruSeq Adapter, Index 7 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACGGAATCTCGTA20530194.9127058770828915TruSeq Adapter, Index 7 (97% over 37bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCACAC2137050.5113785159621023Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACAC1999190.478389750041541Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTACAC726490.1738430912057779Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCACA496530.11881555159245813Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG494310.11828432382266527Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTGAACTCCAG424860.10166550913252323Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTACACTCTTTCCCTAGA419880.10047383602731219Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)