FastQCFastQC Report
Thu 25 Aug 2022
EGAF00005312449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41789984
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC21703755.193529148037003Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG21638915.17801346849044Illumina Single End PCR Primer 1 (100% over 50bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCGTC2560820.612783197045493Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGAAGAGCACACGTCTAGGGAAAGAGTGTAGATCGGAAGAGCGTCG1979650.47371398850021096No Hit
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCGTCG645110.15436952548246968Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTGATCGGAAGAGCGTCG634990.15194789258593638Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTGATCGGAAGAGCACAC598320.14317306271282612Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGATCGGAAGAGCACACGTCTAGGGAAAGAGTGTAGATCGGAAGAGCGTC450170.10772198429173843Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AGACGTGTGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAG418170.10006464706949876Illumina Single End PCR Primer 1 (100% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)