FastQCFastQC Report
Sat 27 Aug 2022
EGAF00005312450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39269148
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGTACAATCTCGTAT16350024.16357900100099TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGTACAATCTCGTA9542802.4301011063443494TruSeq Adapter, Index 7 (97% over 36bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCACAC1609950.40997833719234245Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACAC696490.17736315542165568Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTACACTCTTTCCCTAGA615500.15673882203912345Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTACAC538650.13716875140759355Illumina Single End PCR Primer 1 (100% over 33bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGTACAATCTCGTAT467860.11914187697680631TruSeq Adapter, Index 1 (97% over 34bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCACA395250.10065153438011946Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)