FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39269148
Sequences flagged as poor quality0
Sequence length100
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG17123744.360608995132769Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC10098472.5716040490616194Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTAGGGAAAGAGTGTAGATCGGAAGAGCGTCG1571690.4002353195949146No Hit
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCGTC974670.24820248200954093Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
NATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG742620.18911029085734174Illumina Single End PCR Primer 1 (98% over 50bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTGATCGGAAGAGCACAC475840.1211740066272892Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
NGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC440690.11222295935730514Illumina Single End PCR Primer 1 (98% over 50bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCGTCG397100.10112264213117128Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)