FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30011788
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG16597675.530383594606226Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC9275063.090472317077543Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTAGGGAAAGAGTGTAGATCGGAAGAGCGTCG1979120.6594475477435733No Hit
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCGTC993660.33108990374049024Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCGTCG506510.1687703511700136Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AGATCGGAAGAGCACACGTCTAGGGAAAGAGTGTAGATCGGAAGAGCGTC473420.1577446835223546Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTGATCGGAAGAGCACAC439150.1463258370344346Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGAAGAGCACACGTCTAGGGAAAGAGTGTAGATCGGAAGAGCACAC356490.11878332607174222No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)