FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46408968
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTAT8165911.759554317174215TruSeq Adapter, Index 5 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTA6930361.4933234455892233TruSeq Adapter, Index 5 (97% over 37bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCACAC721580.15548287994682408Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACAC555140.11961912189040705Illumina Single End PCR Primer 1 (100% over 33bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG489900.10556149406295784No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)