FastQCFastQC Report
Sat 27 Aug 2022
EGAF00005312457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47754894
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG21557074.514106972994223Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC20305444.252012369664144Illumina Single End PCR Primer 1 (100% over 50bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCGTC1987190.4161227957075981Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGAAGAGCACACGTCTAGGGAAAGAGTGTAGATCGGAAGAGCGTCG1781890.3731324374837896No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA788800.16517678795392154No Hit
AGATCGGAAGAGCACACGTCTAGGGAAAGAGTGTAGATCGGAAGAGCGTC694690.14546990723086936Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCGTCG539500.11297271437771383Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTGATCGGAAGAGCGTCG494510.10355169042988556Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)