FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37699601
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCTCGAATCTCGTAT7451981.9766734401247377TruSeq Adapter, Index 15 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCTCGAATCTCGTA4684911.242694849741248TruSeq Adapter, Index 15 (97% over 36bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCACAC575760.1527231017643927Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG507520.13462211443564084No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACAC414010.10981813839356018Illumina Single End PCR Primer 1 (100% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)