FastQCFastQC Report
Sat 27 Aug 2022
EGAF00005312461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39651400
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG9086792.2916693988106345Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC7396141.8652910111622794Illumina Single End PCR Primer 1 (100% over 50bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA2166540.5463968485349824No Hit
GATCGGAAGAGCACACGTCTAGGGAAAGAGTGTAGATCGGAAGAGCGTCG695840.1754893900341476No Hit
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCGTC690610.1741703949923584Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG496240.12515068824808204No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)