FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40007333
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGCAATCTCGTAT7044271.7607447114757688TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGCAATCTCGTA5927501.481603385059434TruSeq Adapter, Index 14 (97% over 38bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG765820.19141990794537592No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTACAC615850.15393427999811934Illumina Single End PCR Primer 1 (100% over 33bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA590240.14753295352129572No Hit
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCACAC580340.145058407167506Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACAC494310.12355484930725075Illumina Single End PCR Primer 1 (100% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)