FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40007333
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG7345141.835948424755032Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC6211801.5526653576233138Illumina Single End PCR Primer 1 (100% over 50bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA1166360.29153655406122675No Hit
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCGTC851750.21289847038791615Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGAAGAGCACACGTCTAGGGAAAGAGTGTAGATCGGAAGAGCGTCG588990.1472205107998576No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)