FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41622680
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTAT16696654.011430787253488TruSeq Adapter, Index 13 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTA11824532.8408862668141506TruSeq Adapter, Index 13 (97% over 38bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCACAC1534720.3687220524963794Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACAC995490.23917008707752602Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTACAC961280.2309510103626196Illumina Single End PCR Primer 1 (100% over 33bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG882600.21204785467922777No Hit
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTACACTCTTTCCCTAGA741720.17820092315055158Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA639440.153627781776666No Hit
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCACA579800.13929905522662164Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTAT476040.11437033847892543TruSeq Adapter, Index 13 (97% over 38bp)
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT453330.10891417851997998No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)