FastQCFastQC Report
Thu 25 Aug 2022
EGAF00005312474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37773533
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGAGGAATCTCGTAT12299523.2561211576370153TruSeq Adapter, Index 25 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGAGGAATCTCGTA8109132.146775627262613TruSeq Adapter, Index 25 (97% over 38bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCACAC1160960.30734747528117107Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACAC663560.17566797365764014Illumina Single End PCR Primer 1 (100% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGAGGAAACTCGTAT435810.11537443426327106TruSeq Adapter, Index 25 (97% over 38bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTACAC434250.11496144668278713Illumina Single End PCR Primer 1 (100% over 33bp)
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCACA387490.10258240869340976Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)