FastQCFastQC Report
Sat 27 Aug 2022
EGAF00005312479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69814418
Sequences flagged as poor quality0
Sequence length100
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA4218230.6042061397690087No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1983830.2841576363209101Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1689980.2420674766636313Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG782200.11203989410897904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)