FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48960828
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCATCTCGTAT1397970.2855282594485534TruSeq Adapter, Index 2 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCATCTCGTA1113230.22737156324235366TruSeq Adapter, Index 2 (97% over 38bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG832930.1701217144448619No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA515410.10526987002752485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)