FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51221387
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG1881250.3672782230594419No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA1255050.24502460271136353No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTA1058380.20662853194506428TruSeq Adapter, Index 5 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTAT1049040.20480507488014724TruSeq Adapter, Index 5 (97% over 38bp)
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT867350.1693335637318841No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)