FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51221387
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA2810540.5487043917807224No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1131190.22084329735155359Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1113330.21735647260000984Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG646430.12620314244907113No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)