FastQCFastQC Report
Sat 27 Aug 2022
EGAF00005312505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37837013
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA13879573.6682520367027918No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC10462412.7651257777668654Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG7329591.9371481570175744Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG2460560.6503050333280801No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1222830.32318354517043935No Hit
ACACTCTTTCCCTAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCGTC577230.15255696849008668Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACACACACACACACACACACACACACACACACACACACACACACACACAC466300.12323911509610974No Hit
AGATCGGAAGAGCACACGTCTAGGGAAAGAGTGTAGATCGGAAGAGCGTC432640.11434306402569358Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)