FastQCFastQC Report
Thu 25 Aug 2022
EGAF00005312522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48064183
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTA1948950.40548905200365104TruSeq Adapter, Index 20 (97% over 38bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG1947640.4052164997790558No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTAT1841080.38304614477687055TruSeq Adapter, Index 20 (97% over 38bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA1051390.2187470865779618No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT912260.1898003758848871No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT486770.10127499722610493No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)