FastQCFastQC Report
Sat 27 Aug 2022
EGAF00005312526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53672027
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACGCAAATCTCGTA2516870.4689351494028724TruSeq Adapter, Index 18 (97% over 40bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG2457500.45787352134101444No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACGCAAATCTCGTAT2150460.4006668129005077TruSeq Adapter, Index 18 (97% over 40bp)
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1123810.20938467630447424No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA968280.18040682532821053No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT537380.1001229187785287No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)