FastQCFastQC Report
Fri 26 Aug 2022
EGAF00005312540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65586174
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATGTACATCTCGTAT2353000.3587646384129679TruSeq Adapter, Index 2 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATGTACATCTCGTA1667190.2541983924843672TruSeq Adapter, Index 2 (97% over 40bp)
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG1178670.17971318162269995No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG674910.10290431028954364No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)