FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005312734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12526852
Sequences flagged as poor quality0
Sequence length30-75
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTAC127550.042.3038377
TTCGTAC142300.042.205028
GACGCAA175400.042.0696687
CGCAATT177300.041.789949
GCTACCT126700.041.7857259
AGCTACC127250.041.7580768
TGATGCC126550.041.6811147
ATGCACT171200.041.6240929
TATCGTC77900.041.6157078
GATCCTA132750.041.591968
CTGTGAG98650.041.5875558
GTATGCA173950.041.587367
GTGCTAG128950.041.391727
TCGGATT86650.041.3563210
ACGCAAT179000.041.3326458
ATAGCTC87950.041.311088
CCGATTC111000.041.2855077
GATGCCA127300.041.2829748
TATGCAC173250.041.2813648
TATCCGG81850.041.2719968
Produced by FastQC (version 0.11.5)