FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005312735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12526852
Sequences flagged as poor quality0
Sequence length75-120
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG463460.3699732382884383No Hit
GTAACTTTTTTGTGTATGTACTTAGCTGTACTATAAGTAGTTGGTTTGTA209850.16752013993619466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGCG17950.068.2855456
CCGATAG14800.061.3932884
CGATAGC18050.061.089075
CTCGGCG73700.059.522358114
TATAGCG10500.051.381776
GCGACTG11500.049.75574114
CGATTGG21600.048.2007455
AGCGCCC32250.048.084354114
CTCTCGG111500.040.79276112-113
GGTTTCT158800.039.2377480-81
CCCGTAC32450.039.229123
TCTATGA140800.038.8106104-105
TGCTGTT179800.038.788074114
ATAGCGT154550.038.463047
TAGCGTA157650.038.3402148
CGTACTA25700.037.933155
GGCGAGC124900.037.7839582-83
AGGTTTC138250.037.51223878-79
AAGGTTT168550.037.25668778-79
AGCGTAT162750.037.228559
Produced by FastQC (version 0.11.5)