FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005312748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34017214
Sequences flagged as poor quality0
Sequence length30
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGAC288100.023.3171887
TGACATT406300.023.29117610
TAATCCG167850.023.2068888
AGTCAGG181250.023.1064917
TCTCGTC265800.023.1063797
GAAGTTT407500.023.07460211
CCTGACA295300.023.0493857
GACAATT328700.023.03986510
AGTGACA289450.023.0303368
AGAAGTT320800.023.01622210
ATAGCTG179250.023.0161827
GATCCTT358450.023.009289
CAAGAAG199350.023.0012028
GCGTAGG299850.022.999958
TGACAAT295250.022.9922549
CAAGTTT479300.022.98779311
ACACCGT164200.022.977147
AATCTTT331050.022.96256611
ACGTGTT267650.022.9508810
ACAATTT601100.022.93921311
Produced by FastQC (version 0.11.5)