FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005312750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30669846
Sequences flagged as poor quality0
Sequence length30
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA449000.14639786583864817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGAAT122150.023.1941817
TATAGGC218300.023.1753468
TTGTGTC253900.023.078288
GAAGTTT393650.023.06972711
ATACACG188000.023.0678377
TGGTAGC180150.023.0605327
CTTGCTG265900.023.0160738
TGCTGTT391500.023.0129310
AGGAACG129550.022.9995148
ACAATTT520100.022.99583811
TGCGACG117050.022.9952038
CTGTAAC191700.022.929488
TGACATT231450.022.9059310
GCATAAT185800.022.8824217
TTCAGAC180800.022.8780737
CGATTCT220750.022.8692118
GCTAATG209550.022.8658947
AGGAGAC118700.022.8573787
CCGATTC222450.022.850737
GTGACTA115550.022.836778
Produced by FastQC (version 0.11.5)