FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005312754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28525325
Sequences flagged as poor quality0
Sequence length30
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGCC184950.023.2858357
TACACGG217150.023.2649758
AAGAGAC189750.023.1714928
ATACACG220200.023.1712067
GCGTATA165150.023.1494067
TTGTGCA357600.023.1308218
AATCTTT356200.023.12703911
CTGATTT280800.023.12351611
GATGGAT213000.023.1211179
TGACATT175250.023.10971310
AGAGACT189300.023.1061739
TCGGAAT226500.023.0988837
ATCCACG191100.023.095718
CGGAATC225050.023.0774888
TAGAACG203550.023.0390078
AAGTTTT726250.023.03136412
ATCTCGC184500.023.0308448
AACCTTT409400.023.00300611
GACAAAC192250.022.9946067
CTATGGT246300.022.9865518
Produced by FastQC (version 0.11.5)