FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005312760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32289824
Sequences flagged as poor quality0
Sequence length30
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG268250.023.2240167
TCGACTC210900.023.1387547
TGACATT238800.023.09821910
CCGATTC186950.023.092887
TACTGCA220400.023.06697
TAAGCCG160200.023.0316477
AACTCCG234400.023.0150058
GACAATT225050.023.01127810
GCAGATT208800.023.00347310
AGTGCAG210700.022.9898747
GAAGTTT480450.022.97365611
TCCGTTT349250.022.93599511
AGCCGTT159400.022.9212689
ACTCCGT234850.022.873699
AGTGTTT310200.022.8487711
CTCCGTT234900.022.84831810
AGCACAT318750.022.8459558
AAGCACA319300.022.8441817
GGAAGAT166050.022.8416778
CCCTACA121700.022.834448
Produced by FastQC (version 0.11.5)