FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005312762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60715985
Sequences flagged as poor quality0
Sequence length30
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGCT657300.023.3476759
ACTAGCG280600.023.3305997
ATAGGGC386800.023.2717138
TTGATGG677400.023.2643577
TCACCCG452100.023.1590087
CGGTATT761650.023.143699
GCGGTAT768100.023.1415448
TAGAGGG512100.023.1401147
ACGATAG536550.023.1210527
CCGAGAT409900.023.11957
TGATGGC670000.023.0896328
CGATAGG533650.023.0622488
GAACAGC450050.023.0592087
AGAAGTT800700.023.05190710
TTGTGTC512200.023.0465038
AGGTAAA418550.023.0172447
AGAATGT349550.023.0123739
CAAGAAG380150.022.9781048
TCCGTTT782700.022.96422411
TACACGG362550.022.9550888
Produced by FastQC (version 0.11.5)