FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005312764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28011363
Sequences flagged as poor quality0
Sequence length30
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCAGAC298550.023.3867638
AGACTTT589500.023.33402311
TGACATT330100.023.32147810
CGAGAAT182250.023.3196547
TGATTCG194050.023.2866947
GGCGATT203500.023.260757
CAACGAC121050.023.1848478
TCGACTC173900.023.1628977
CATAACT236200.023.1441429
GACATTT332300.023.13819311
CTGATTT392100.023.1376711
GAAGTTT221200.023.13508811
CAGTGAC154050.023.133217
ACATTTT984500.023.12249212
CAGGTTT348250.023.10175111
CACACTG236750.023.1007187
TTGACAT182050.023.088029
ATAACTT236850.023.08062610
TTCCGAT209550.023.0643188
ACTCATT175450.023.04012910
Produced by FastQC (version 0.11.5)