FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005312778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24942023
Sequences flagged as poor quality0
Sequence length30
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCG162900.023.3347537
AGGTCCT340500.023.2328459
AAGGTCC343400.023.2323658
GGAGACT181800.023.2187758
GGGTTAT307800.023.214127
GTTATGT305300.023.2075659
AATCGGC102750.023.1101677
AGGAGAC183850.023.1100777
GGTTATG308100.023.082388
CAATACG186650.023.0591077
TTGTGTC239300.023.0248368
GGTCCTT340000.023.01988810
ATCGACG131700.023.00467
TATCGCT140150.023.0044528
AATACGC184850.023.0044368
AGACAAC197050.022.9990377
ACAGAAG176950.022.9940957
ACGTGTT254750.022.96123110
AGTTTCG122000.022.9256447
CCGTATT190050.022.89257410
Produced by FastQC (version 0.11.5)