FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005312779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24942023
Sequences flagged as poor quality0
Sequence length120
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2282470.9151102137946068No Hit
GTAACTTTTTTGTGTATGTACTTAGCTGTACTATAAGTAGTTGGTTTGTA464110.18607552402625882No Hit
GCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGG422010.1691963799407931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAG60650.088.386384
GATAGCG83800.085.266186
CGATAGC74600.085.079385
TATAGCG38600.080.451196
TCCGATA51900.073.093013
GCTCCGA46500.069.071291
CATAGCG62100.056.4295736
CTCGGCG470300.056.087498114
CCCCCGT135300.054.000811
TAGCGTA700450.052.5114978
ATAGCGT690850.052.2376757
TCGATAG14700.051.9294784
AGCGTAT712650.051.6732989
CTAGCGT15800.050.1228375
CCCGATA23950.048.7547953
CCCGTAC35950.047.215693
ACCGGAA84350.046.391743
CTCGGCC194300.045.877945114
TACCCGT16400.044.099261
CTACCGG50200.043.787991
Produced by FastQC (version 0.11.5)