FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005312780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30957047
Sequences flagged as poor quality0
Sequence length30
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG338100.023.3163077
AAACTGC238900.023.2843848
GAAGTTT643000.023.25850711
TCCGTTT324650.023.1917611
GTAGAAG344400.023.1858168
GAAACTG243150.023.158797
AGAAGTT527450.023.08755910
CAGATGA306750.023.082278
ACAAGGG168550.023.0151828
CGAACTT172600.023.0103539
GATGATT309150.022.9883910
GGCACTT188850.022.9872849
AGGAGAC167000.022.9846217
ATGATTT333200.022.97476211
CACCCGA342300.022.9354638
TCGACTC188350.022.9274547
TAGAAGT349350.022.925919
AACTGCT241350.022.8987989
AGTCGTT140650.022.87995110
AGATGAT310600.022.8772099
Produced by FastQC (version 0.11.5)